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in primary antibodies in 10% goat or donkey serum (Sigma)

The secondary antibodies used are as follows: Goat Anti-Mouse IgG H&L (Alexa Fluor 488) (Abcam, ab150113), Goat Anti-Mouse IgG H&L (Alexa Fluor 647) (Abcam, ab150115), Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (Abcam, ab150077), Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) (Abcam, ab150075)

Pharmacokinetics and Best Injection Methods SC vs

Addition of a large quantity of the enzyme would have increased the enzymatic contribution of the total rate of reaction mixture but that would have made the rate too fast to measure accurately and conveniently, especially at high MBB concentration